Polr2m^tm1.1Rgr
Targeted Allele Detail

Summary

• Symbol: Polr2m^tm1.1Rgr
• Name: polymerase (RNA) II (DNA directed) polypeptide M; targeted mutation 1.1, Robert G Roeder
• MGI ID: MGI:6503875
• Synonyms: Gdown1^f
• Gene: Polr2m Location: Chr9:71385719-71393217 bp, - strand Genetic Position: Chr9, 39.85 cM
• Alliance: Polr2m^tm1.1Rgr page
• IMPC: Polr2m gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:291184
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 71388346 of Chromosome 9 upstream of the exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 71391587. The critical exons, 2 and 3, are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:291184)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Polr2m Mutation: 20 strains or lines available

References

• Original: J:291184 Jishage M, et al., Transcriptional down-regulation of metabolic genes by Gdown1 ablation induces quiescent cell re-entry into the cell cycle. Genes Dev. 2020 Jun 1;34(11-12):767-784
• All: 1 reference(s)