Eps15^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Eps15^{tm1c(KOMP)Wtsi}
• Name: epidermal growth factor receptor pathway substrate 15; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6505482
• Synonyms: Eps15^flp
• Gene: Eps15 Location: Chr4:109137465-109245014 bp, + strand Genetic Position: Chr4, 51.29 cM, cytoband C7
• Alliance: Eps15^{tm1c(KOMP)Wtsi} page
• IMPC: Eps15 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:272122
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt0 cassette was inserted at position 109178803 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 109179632. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:272122)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Eps15 Mutation: 66 strains or lines available

References

• Original: J:272122 Milesi C, et al., Redundant and nonredundant organismal functions of EPS15 and EPS15L1. Life Sci Alliance. 2019 Feb;2(1)
• All: 1 reference(s)