Prdm9^em3Kpgn
Endonuclease-mediated Allele Detail

Summary

• Symbol: Prdm9^em3Kpgn
• Name: PR domain containing 9; endonuclease-mediated mutation 3, Kenneth Paigen
• MGI ID: MGI:6506065
• Synonyms: Prdm9^EK
• Gene: Prdm9 Location: Chr17:15763341-15784616 bp, - strand Genetic Position: Chr17, 8.95 cM
• Alliance: Prdm9^em3Kpgn page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Hypomorph)
• Mutation: Single point mutation
• Mutation details: Using CRISPR/Cas9 technology, a G-to-A substitution (C-to-T on forward strand) was created in glutamic acid codon 360 (initially reported as 365) to change it to a lysine codom (p.E360K). The mutation is located in the PR/SET domain and leads to the loss of most of the enzyme's histone methyltransferase activity. As this allele was created in the C57BL/6 strain, it is based on the Prdm9^dom2 allele. (J:280261)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prdm9 Mutation: 46 strains or lines available

References

• Original: J:280261 Bhattacharyya T, et al., Prdm9 and Meiotic Cohesin Proteins Cooperatively Promote DNA Double-Strand Break Formation in Mammalian Spermatocytes. Curr Biol. 2019 Mar 18;29(6):1002-1018.e7
• All: 1 reference(s)