Lgr5^{tm3.1(cre/ERT2)Niba}
Targeted Allele Detail

Summary

• Symbol: Lgr5^{tm3.1(cre/ERT2)Niba}
• Name: leucine rich repeat containing G protein coupled receptor 5; targeted mutation 3.1, Nick Barker
• MGI ID: MGI:6507189
• Synonyms: Lgr5-2A-creERT2
• Gene: Lgr5 Location: Chr10:115286219-115423685 bp, - strand Genetic Position: Chr10, 63.51 cM
• Alliance: Lgr5^{tm3.1(cre/ERT2)Niba} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:292580
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Lgr5^{tm3.1(cre/ERT2)Niba} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Lgr5 (MGI:1341817)

• Mutation details: Sequence for the 2A self-cleaving peptide, a cre-ERT2 fusion gene and a loxP site flanked neomycin resistance gene cassette were inserted immediately before the stop codon. The neo cassette was removed through subsequent cre-mediated recombination. This allele expresses the endogenous peptide and the inserted cre-ERT2 fusion peptide. (J:247209, J:292580)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Lgr5 (mouse) Summary of all recombinase alleles driven by Lgr5. Comparative matrix view of recombinase activities Alliance mouse Lgr5 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lgr5 Mutation: 58 strains or lines available

References

• Original: J:247209 Leushacke M, et al., Lgr5-expressing chief cells drive epithelial regeneration and cancer in the oxyntic stomach. Nat Cell Biol. 2017 Jul;19(7):774-786
• All: 5 reference(s)