Igs2^{tm1(CAG-Met)Zsu}
Targeted Allele Detail

Summary

• Symbol: Igs2^{tm1(CAG-Met)Zsu}
• Name: intergenic site 2; targeted mutation 1, Zijie Sun
• MGI ID: MGI:6507851
• Synonyms: H11^L-Met, H11^Met
• Gene: Igs2 Location: Chr11:3195460-3195497 bp Genetic Position: Chr11, Syntenic
• Alliance: Igs2^{tm1(CAG-Met)Zsu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:268934
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: Site-specific PhiC31 integrase-mediated transgenesis is used to insert the targeting construct containing an attB site, the CAG promoter, a loxP flanked PGK-neomycin STOP cassette, a FLAG-tagged mouse Met cDNA, a polyA signal and a second attB site into the intergenic site 2 locus. This construct was microinjected, along with the PhiC31 integrase mRNA into the pronuclei of zygotes of an FVB/N mouse that contains an attP docking site at the H11 locus which is either the Igs2^tm3.1Luo or the Igs2^tm4Luo. The PhiC31 integrase catalyzes recombination between the attB and attP sites. (J:268934)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igs2 Mutation: 72 strains or lines available

Notes

This allele was produced in zygotes collected from mice carrying either the Igs2^tm3.1Luo allele or the Igs2^tm4Luo allele (not specified which is used), which was generated in (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 ES cells.

References

• Original: J:268934 Mi J, et al., Activation of hepatocyte growth factor/MET signaling initiates oncogenic transformation and enhances tumor aggressiveness in the murine prostate. J Biol Chem. 2018 Dec 28;293(52):20123-20136
• All: 2 reference(s)