Gfm1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Gfm1^{tm1c(EUCOMM)Wtsi}
• Name: G elongation factor, mitochondrial 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6508027
• Synonyms: Gfm1^flox
• Gene: Gfm1 Location: Chr3:67337448-67382401 bp, + strand Genetic Position: Chr3, 30.96 cM
• Alliance: Gfm1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Gfm1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:302272
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt1 cassette was inserted at position 67342400 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 67343316. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:302272)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gfm1 Mutation: 33 strains or lines available

References

• Original: J:302272 Almeida L, et al., Ribosome-Targeting Antibiotics Impair T Cell Effector Function and Ameliorate Autoimmunity by Blocking Mitochondrial Protein Synthesis. Immunity. 2021 Jan 12;54(1):68-83.e6
• All: 1 reference(s)