Tpsg1^tm1.2Rls
Targeted Allele Detail

Summary

• Symbol: Tpsg1^tm1.2Rls
• Name: tryptase gamma 1; targeted mutation 1.2, Richard L Stevens
• MGI ID: MGI:6508397
• Synonyms: Prss31^-
• Gene: Tpsg1 Location: Chr17:25588235-25593417 bp, + strand Genetic Position: Chr17, 12.53 cM, cytoband A3.3
• Alliance: Tpsg1^tm1.2Rls page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:243813
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was placed 1.9 kb upstream of the first exon. A second loxP site was placed in the first intron. A puromycin resistance (PuroR) gene flanked by F3 sites was inserted into intron 1 upstream of the second loxP site for positive selection. The puromycin resistance (PuroR) gene was removed via flp1-mediated recombination and exon 1 was removed via cre-mediated recombination in the germline. (J:243813)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tpsg1 Mutation: 28 strains or lines available

References

• Original: J:243813 Hansbro PM, et al., Importance of mast cell Prss31/transmembrane tryptase/tryptase-gamma in lung function and experimental chronic obstructive pulmonary disease and colitis. J Biol Chem. 2014 Jun 27;289(26):18214-27
• All: 1 reference(s)