Lbhd2^{em1(cre/ERT2)Fuki}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Lbhd2^{em1(cre/ERT2)Fuki}
• Name: LBH domain containing 2; endonuclease-mediated mutation 1, Izumi Fukunaga
• MGI ID: MGI:6508398
• Synonyms: Lbhd2-IRES-CreERT2
• Gene: Lbhd2 Location: Chr12:111373243-111378524 bp, + strand Genetic Position: Chr12, 60.98 cM
• Alliance: Lbhd2^{em1(cre/ERT2)Fuki} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:315131
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: C57BL/6JJcl

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Lbhd2^{em1(cre/ERT2)Fuki} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Lbhd2 (MGI:2685744)

• Mutation details: Using CRISPR/cas9 technology an IRES enhancer sequence, a CreER(T2) fusion gene, and a rabbit globin polyadenylation (rGpA) termination sequence were inserted immediately downstream of the LBH domain containing 2 locus of the Lbhd2 gene on chromosome 12. The construct was electroporated into C57BL/6JJcl-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts, and chimeric mice were bred with C57BL/6JJcl for germline transmission. (J:315131)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Lbhd2 (mouse) Summary of all recombinase alleles driven by Lbhd2. Comparative matrix view of recombinase activities Alliance mouse Lbhd2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lbhd2 Mutation: 10 strains or lines available

References

• Original: J:315131 Koldaeva A, et al., Generation and Characterization of a Cell Type-Specific, Inducible Cre-Driver Line to Study Olfactory Processing. J Neurosci. 2021 Jul 28;41(30):6449-6467
• All: 2 reference(s)