Exoc3^{tm2c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Exoc3^{tm2c(EUCOMM)Hmgu}
• Name: exocyst complex component 3; targeted mutation 2c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6509449
• Gene: Exoc3 Location: Chr13:74317607-74356851 bp, - strand Genetic Position: Chr13, 40.15 cM
• Alliance: Exoc3^{tm2c(EUCOMM)Hmgu} page
• IMPC: Exoc3 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:302300
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 74347354 of Chromosome 13 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 74348217. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. (J:302300)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Exoc3 Mutation: 28 strains or lines available

References

• Original: J:302300 Walsh TG, et al., Loss of the exocyst complex component EXOC3 promotes hemostasis and accelerates arterial thrombosis. Blood Adv. 2021 Feb 9;5(3):674-686
• All: 1 reference(s)