Gm15441^em1Gonz
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gm15441^em1Gonz
• Name: predicted gene 15441; endonuclease-mediated mutation 1, Frank Gonzalez
• MGI ID: MGI:6510264
• Synonyms: Gm15441^LSL
• Gene: Gm15441 Location: Chr3:96463084-96474117 bp, - strand Genetic Position: Chr3, 41.93 cM
• Alliance: Gm15441^em1Gonz page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Null/knockout)
• Mutation: Insertion
• Mutation details: A loxP site flanked STOP cassette was inserted into intron 1 using an sgRNA and a donor plasmid with CRISPR/Cas9 technology. This creates a null allele that can only be transcribed full-length after cre-mediated removal of the cassette. Fluorescent in situ hybridization (FISH) experiments on liver confirmed the absence of transcription from this allele. (J:299830)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gm15441 Mutation: 9 strains or lines available

References

• Original: J:299830 Brocker CN, et al., Long non-coding RNA Gm15441 attenuates hepatic inflammasome activation in response to PPARA agonism and fasting. Nat Commun. 2020 Nov 17;11(1):5847
• All: 1 reference(s)