Cmtm6^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cmtm6^{tm1c(EUCOMM)Wtsi}
• Name: CKLF-like MARVEL transmembrane domain containing 6; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6511038
• Synonyms: Cmtm6^flox
• Gene: Cmtm6 Location: Chr9:114560184-114578412 bp, + strand Genetic Position: Chr9, 65.06 cM
• Alliance: Cmtm6^{tm1c(EUCOMM)Wtsi} page
• IMPC: Cmtm6 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:296495
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 114565819 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 114569458. Exon 2 and 3 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by subsequent flp-mediated recombination to remove the lacZ-neo cassette. (J:296495)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cmtm6 Mutation: 13 strains or lines available

References

• Original: J:296495 Eichel MA, et al., CMTM6 expressed on the adaxonal Schwann cell surface restricts axonal diameters in peripheral nerves. Nat Commun. 2020 Sep 9;11(1):4514
• All: 1 reference(s)