Kif9^em1Osb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Kif9^em1Osb
• Name: kinesin family member 9; endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:6511741
• Synonyms: Kif9^-16
• Gene: Kif9 Location: Chr9:110306062-110354242 bp, + strand Genetic Position: Chr9, 59.92 cM
• Alliance: Kif9^em1Osb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:303071
• Parent Cell Line: EGR-G01 (ES Cell)
• Strain of Origin: (129S2/SvPas x C57BL/6NSlc)F1-Tg(CAG-EGFP,Acr-EGFP)2Osb

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 genome editing technology was used to generate a 16bp deletion in exon 2. The 16 bp deletion caused a P15L mutation resulting in a premature stop codon introduced three amino acids later. Western blot analysis revealed the absence of encoded protein in the testes and cauda epididymal spermatozoa from homozygous mutant male mice. Immunofluorescence confirmed the absence of protein in mutant spermatozoa. (J:303071)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Kif9 Mutation: 52 strains or lines available

References

• Original: J:303071 Miyata H, et al., Testis-enriched kinesin KIF9 is important for progressive motility in mouse spermatozoa. FASEB J. 2020 Apr;34(4):5389-5400
• All: 2 reference(s)