Kif9^em2Osb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Kif9^em2Osb
• Name: kinesin family member 9; endonuclease-mediated mutation 2, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:6511743
• Synonyms: Kif9^LD
• Gene: Kif9 Location: Chr9:110306062-110354242 bp, + strand Genetic Position: Chr9, 59.92 cM
• Alliance: Kif9^em2Osb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:303071
• Parent Cell Line: EGR-G01 (ES Cell)
• Strain of Origin: (129S2/SvPas x C57BL/6NSlc)F1-Tg(CAG-EGFP,Acr-EGFP)2Osb

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 genome editing technology was used to generate a large 902 bp deletion. Western blot analysis confirmed the absence of protein expression in the testes and cauda epididymal spermatozoa from homozygous mutant male mice. (J:303071)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Kif9 Mutation: 52 strains or lines available

References

• Original: J:303071 Miyata H, et al., Testis-enriched kinesin KIF9 is important for progressive motility in mouse spermatozoa. FASEB J. 2020 Apr;34(4):5389-5400
• All: 2 reference(s)