Slc25a28^tm1.2Dwrd
Targeted Allele Detail

Summary

• Symbol: Slc25a28^tm1.2Dwrd
• Name: solute carrier family 25, member 28; targeted mutation 1.2, Diane Ward
• MGI ID: MGI:6514336
• Synonyms: Mfrn2^-
• Gene: Slc25a28 Location: Chr19:43652240-43663320 bp, - strand Genetic Position: Chr19, 36.67 cM
• Alliance: Slc25a28^tm1.2Dwrd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:300667
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted into intron 3 and an FRT site flanked neomycin resistance gene and a second loxP site into the 3' UTR in exon 4. The neo cassette was removed through subsequent flp-mediated recombination and exon 4 was deleted through cre-mediated recombination. RT-qPCR confirmed the lack of transcription from this allele. (J:300667)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc25a28 Mutation: 18 strains or lines available

References

• Original: J:300667 Seguin A, et al., The mitochondrial metal transporters mitoferrin1 and mitoferrin2 are required for liver regeneration and cell proliferation in mice. J Biol Chem. 2020 Aug 7;295(32):11002-11020
• All: 1 reference(s)