Slc17a7^{em1(flpo)Tasic}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Slc17a7^{em1(flpo)Tasic}
• Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7; endonuclease-mediated mutation 1, Bosiljka Tasic
• MGI ID: MGI:6514364
• Synonyms: Slc17a7^em1(flpo)Hze
• Gene: Slc17a7 Location: Chr7:44813373-44825566 bp, + strand Genetic Position: Chr7, 29.16 cM
• Alliance: Slc17a7^{em1(flpo)Tasic} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Endonuclease-mediated (Recombinase)
• Mutation: Insertion
• Slc17a7^{em1(flpo)Tasic} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Slc17a7 (MGI:1920211)

• Mutation details: CRISPR/cas9 genome editing is used to insert an internal ribosome entry site 2 sequence), a FlpO recombinase gene, a woodchuck hepatitis virus post-transcriptional regulatory element, a bovine growth hormone polyA sequence, an attB site, a PGK/gb2 promoter-Neomycin resistance gene-PGK polyA cassette and an attP site following the STOP codon. The guide vector contained the Slc17a7 guide RNA sequences designed for the endogenous stop codon and also a DNA sequence encoding a human codon-optimized Streptococcus pyogenes CRISPR associated protein 9 (SpCas9). Of note, the guide vector is designed so the SpCas9 protein is only expressed in embryonic stem (ES) cells and the SpCas9 DNA sequence does not integrate into the genome. (J:101977)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Slc17a7 (mouse) Summary of all recombinase alleles driven by Slc17a7. Alliance mouse Slc17a7 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Slc17a7 Mutation: 40 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 2 reference(s)