Sncg^{em1(flpo)Tasic}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Sncg^{em1(flpo)Tasic}
• Name: synuclein, gamma; endonuclease-mediated mutation 1, Bosiljka Tasic
• MGI ID: MGI:6514365
• Synonyms: Sncg^em1(flpo)Hze
• Gene: Sncg Location: Chr14:34092231-34096626 bp, - strand Genetic Position: Chr14, 20.81 cM, cytoband B-C
• Alliance: Sncg^{em1(flpo)Tasic} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:313881
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Endonuclease-mediated (Recombinase)
• Mutation: Insertion
• Sncg^{em1(flpo)Tasic} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Sncg (MGI:1298397)

• Mutation details: CRISPR/cas9 genome editing is used to insert an internal ribosome entry site 2 sequence, a FlpO recombinase gene, a bovine growth hormone polyA sequence, an attB site, a PGK/gb2 promoter-Neomycin resistance gene-PGK polyA cassette and an attP site following the STOP codon. The guide vector contained the Sncg guide RNA sequences designed for the endogenous stop codon and also a DNA sequence encoding a human codon-optimized Streptococcus pyogenes CRISPR associated protein 9 (SpCas9). Of note, the guide vector is designed so the SpCas9 protein is only expressed in embryonic stem (ES) cells and the SpCas9 DNA sequence does not integrate into the genome (J:313881)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Sncg (mouse) Summary of all recombinase alleles driven by Sncg. Alliance mouse Sncg gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sncg Mutation: 16 strains or lines available

References

• Original: J:313881 Lee BR, et al., Scaled, high fidelity electrophysiological, morphological, and transcriptomic cell characterization. Elife. 2021 Aug 13;10:e65482
• All: 3 reference(s)