Prss59^em1Fcw
Endonuclease-mediated Allele Detail

Summary

• Symbol: Prss59^em1Fcw
• Name: serine protease 59; endonuclease-mediated mutation 1, Fengchao Wang
• MGI ID: MGI:6514738
• Synonyms: Tryx5^-
• Gene: Prss59 Location: Chr6:40897371-40917491 bp, - strand Genetic Position: Chr6, 18.97 cM
• Alliance: Prss59^em1Fcw page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology deleted 330 bp which includes exon 4 resulting in a frameshift mutation. Sequencing of the RT-PCR amplicon confirmed deletion of exon 4 and a splicing of exon 3 to exon 5. The frameshift mutation occurred from valine 14 to the last amino acid resulting in a new stop codon at the position of the 40th codon. (J:303285)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prss59 Mutation: 23 strains or lines available

References

• Original: J:303285 Zhang H, et al., Male fertility in Mus musculus requires the activity of TRYX5 in sperm migration into the oviduct. J Cell Physiol. 2020 Sep;235(9):6058-6072
• All: 1 reference(s)