Ttll8^tm1.2Ics
Targeted Allele Detail

Summary

• Symbol: Ttll8^tm1.2Ics
• Name: tubulin tyrosine ligase-like family, member 8; targeted mutation 1.2, Mouse Clinical Institute
• MGI ID: MGI:6514903
• Gene: Ttll8 Location: Chr15:88774836-88838621 bp, - strand Genetic Position: Chr15, 44.39 cM
• Alliance: Ttll8^tm1.2Ics page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:300009
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 88822888 of Chromosome 15 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 88823661. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:300009)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ttll8 Mutation: 38 strains or lines available

References

• Original: J:300009 Gadadhar S, et al., Tubulin glycylation controls axonemal dynein activity, flagellar beat, and male fertility. Science. 2021 Jan 8;371(6525)
• All: 1 reference(s)