Ssmem1^em1Osb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ssmem1^em1Osb
• Name: serine-rich single-pass membrane protein 1; endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:6515722
• Synonyms: Ssmem1^-5
• Gene: Ssmem1 Location: Chr6:30509848-30520253 bp, + strand Genetic Position: Chr6, 12.52 cM, cytoband A3.3
• Alliance: Ssmem1^em1Osb page

Mutation origin

• Strain of Origin: (C57BL/6NJcl x DBA/2NJcl)F2

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: CRISPR-targeting of exon 2 produced a 6 bp deletion and 1 bp insertion that leads to a frame shift and a stop codon instead of L77. Western blot analysis confirmed the absence of protein expression in the sperm and testes. (J:296963)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ssmem1 Mutation: 19 strains or lines available

References

• Original: J:296963 Nozawa K, et al., Knockout of serine-rich single-pass membrane protein 1 (Ssmem1) causes globozoospermia and sterility in male mice. Biol Reprod. 2020 Aug 4;103(2):244-253
• All: 1 reference(s)