Ptpra^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Ptpra^{tm1c(EUCOMM)Hmgu}
• Name: protein tyrosine phosphatase receptor type A; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6682153
• Synonyms: Ptpra^f
• Gene: Ptpra Location: Chr2:130292198-130396220 bp, + strand Genetic Position: Chr2, 63.23 cM, cytoband G
• Alliance: Ptpra^{tm1c(EUCOMM)Hmgu} page
• IMPC: Ptpra gene page

Mutation origin

• Mutant Cell Line: HEPD0511_4_G01
• Germline Transmission: Earliest citation of germline transmission: J:300194
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 130359174 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 130360024. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:300194)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptpra Mutation: 45 strains or lines available

References

• Original: J:300194 Aschner Y, et al., Protein tyrosine phosphatase-alpha amplifies transforming growth factor-beta-dependent profibrotic signaling in lung fibroblasts. Am J Physiol Lung Cell Mol Physiol. 2020 Aug 1;319(2):L294-L311
• All: 2 reference(s)