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Tmc6tm1.1Les
Targeted Allele Detail
Summary
Symbol: Tmc6tm1.1Les
Name: transmembrane channel-like gene family 6; targeted mutation 1.1, Lawrence E Samelson
MGI ID: MGI:6690848
Synonyms: Tmc6delta
Gene: Tmc6  Location: Chr11:117656811-117673019 bp, - strand  Genetic Position: Chr11, 82.96 cM
Alliance: Tmc6tm1.1Les page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:300461
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129S6/SvEv
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA 1.6-kb fragment containing exons 6-8 was replaced with a nuclear localization signal and LacZ reporter gene, the Engrailed-2 intron and splice acceptor site (En-2/SA), and an internal ribosome entry site followed by a loxP-flanked phosphoglycerine kinase (PGK)-neomycin resistance gene. The loxP-flanked PGK-neomycin resistance gene was removed via Cre-mediated recombination in the germline. Western blot analysis using a polyclonal anti-TMC6 antibody confirmed the complete absence of protein expression in cell lysates from homozygous mutant thymocytes. (J:300461)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Tmc6 Mutation:  37 strains or lines available
References
Original:  J:300461 Wu CJ, et al., Expression of a TMC6-TMC8-CIB1 heterotrimeric complex in lymphocytes is regulated by each of the components. J Biol Chem. 2020 Nov 20;295(47):16086-16099
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory