Tmc6tm1.1Les
Targeted Allele Detail
|
Symbol: |
Tmc6tm1.1Les |
Name: |
transmembrane channel-like gene family 6; targeted mutation 1.1, Lawrence E Samelson |
MGI ID: |
MGI:6690848 |
Synonyms: |
Tmc6delta |
Gene: |
Tmc6 Location: Chr11:117656811-117673019 bp, - strand Genetic Position: Chr11, 82.96 cM
|
Alliance: |
Tmc6tm1.1Les page
|
|
Germline Transmission: |
Earliest citation of germline transmission:
J:300461
|
Parent Cell Line: |
Not Specified (ES Cell)
|
Strain of Origin: |
129S6/SvEv
|
|
Allele Type: |
|
Targeted (Null/knockout, Reporter) |
Mutations: |
|
Insertion, Intragenic deletion
|
|
|
Mutation details: A 1.6-kb fragment containing exons 6-8 was replaced with a nuclear localization signal and LacZ reporter gene, the Engrailed-2 intron and splice acceptor site (En-2/SA), and an internal ribosome entry site followed by a loxP-flanked phosphoglycerine kinase (PGK)-neomycin resistance gene. The loxP-flanked PGK-neomycin resistance gene was removed via Cre-mediated recombination in the germline. Western blot analysis using a polyclonal anti-TMC6 antibody confirmed the complete absence of protein expression in cell lysates from homozygous mutant thymocytes.
(J:300461)
|
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
Carrying any Tmc6 Mutation: |
37 strains or lines available
|
|
Original: |
J:300461 Wu CJ, et al., Expression of a TMC6-TMC8-CIB1 heterotrimeric complex in lymphocytes is regulated by each of the components. J Biol Chem. 2020 Nov 20;295(47):16086-16099 |
All: |
1 reference(s) |
|