Dis3^tm1.1Uba
Targeted Allele Detail

Summary

• Symbol: Dis3^tm1.1Uba
• Name: DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease; targeted mutation 1.1, Uttiya Basu
• MGI ID: MGI:6693492
• Synonyms: Dis3^C, Dis3^COIN
• Gene: Dis3 Location: Chr14:99314070-99337217 bp, - strand Genetic Position: Chr14, 49.25 cM, cytoband E2.1
• Alliance: Dis3^tm1.1Uba page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:302856
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac or C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change, Reporter)
• Mutation: Insertion
• Mutation details: Lox2372 and loxP sites were inserted in intron 2, an mCherry and neo cassette flanked by FRT3 and FRT sites was inserted in intron 3, followed by an antisense oriented conditional inversion (COIN) module consisting of a splice acceptor site and a T2A-GFP-pA cassette, and a second set of lox2372 and loxP sites. The two lox sites are in reverse complementation orientation, thus cre-mediated recombination will irreversibly invert the intervening sequence and also delete exon 3. Flp-mediated recombination removed the mCherry and neo selection cassette. (J:302856)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dis3 Mutation: 43 strains or lines available

References

• Original: J:302856 Laffleur B, et al., Noncoding RNA processing by DIS3 regulates chromosomal architecture and somatic hypermutation in B cells. Nat Genet. 2021 Feb;53(2):230-242
• All: 1 reference(s)