Ifnl3^em1Mtba
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ifnl3^em1Mtba
• Name: interferon lambda 3; endonuclease-mediated mutation 1, Megan T Baldridge
• MGI ID: MGI:6696076
• Synonyms: Ifnl2^-Ifnl3^-
• Gene: Ifnl3 Location: Chr7:28222261-28223747 bp, + strand Genetic Position: Chr7, 16.81 cM
• Alliance: Ifnl3^em1Mtba page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Ifnl3^em1Mtba involves 1 genes/genome features (Ifnl2) View all
• Mutation details: CRISPR/Cas9 technology was used to target a construct containing the coding sequence of tdTomato, an orange fluorescent protein, downstream of self-cleaving peptide T2A to exon 1 of Ifnl3. Inadvertently, a 7.2-kb deletion was introduced 5' of Ifnl3, resulting in removal of exon 1 of Ifnl2. Ifnl2 and Ifnl3 are adjacent genes in an inverted orientation, with the genes and flanking genomic regions sharing high sequence identity. Due to the sequence similarity, the gRNAs used disrupted the Ifnl3 locus with insertion of the reporter gene and also unintentionally caused a large deletion in Ifnl2. The reporter is not functional. (J:287260)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ifnl3 Mutation: 14 strains or lines available

References

• Original: J:287260 Peterson ST, et al., Disruption of Type III Interferon (IFN) Genes Ifnl2 and Ifnl3 Recapitulates Loss of the Type III IFN Receptor in the Mucosal Antiviral Response. J Virol. 2019 Nov 15;93(22)
• All: 2 reference(s)