Arg1^{em2(cre/ERT2)Hzhu}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Arg1^{em2(cre/ERT2)Hzhu}
• Name: arginase, liver; endonuclease-mediated mutation 2, Hao Zhu
• MGI ID: MGI:6704914
• Synonyms: Arg1.2-CreER
• Gene: Arg1 Location: Chr10:24791105-24803368 bp, - strand Genetic Position: Chr10, 12.26 cM
• Alliance: Arg1^{em2(cre/ERT2)Hzhu} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Arg1^{em2(cre/ERT2)Hzhu} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Arg1 (MGI:88070)

• Mutation details: The allele was generated using CRISPR/Cas9 endonuclease-mediated genome editing to have an IRES enhancer sequence and a CreER(T2) fusion gene inserted immediately downstream of the mouse Arg1 gene. These sequences and Cas9 nuclease were introduced into single-cell C57BL/6J zygotes and transferred to pseudopregnant females. Progeny were screened by DNA sequencing to identify correctly targeted pups, which were then bred to C57BL/6J mice for germline transmission. (J:304477)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Arg1 (mouse) Summary of all recombinase alleles driven by Arg1. Alliance mouse Arg1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Arg1 Mutation: 28 strains or lines available

References

• Original: J:304477 Wei Y, et al., Liver homeostasis is maintained by midlobular zone 2 hepatocytes. Science. 2021 Feb 26;371(6532)
• All: 2 reference(s)