Tert^{em1(cre/ERT2)Hzhu}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tert^{em1(cre/ERT2)Hzhu}
• Name: telomerase reverse transcriptase; endonuclease-mediated mutation 1, Hao Zhu
• MGI ID: MGI:6704917
• Synonyms: Tert-CreER
• Gene: Tert Location: Chr13:73775030-73797962 bp, + strand Genetic Position: Chr13, 40.12 cM
• Alliance: Tert^{em1(cre/ERT2)Hzhu} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tert^{em1(cre/ERT2)Hzhu} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tert (MGI:1202709)

• Mutation details: The allele was generated using CRISPR/Cas9 endonuclease-mediated genome editing to have an IRES enhancer sequence and a CreER(T2) fusion gene inserted immediately downstream of the mouse Tert gene. These sequences and Cas9 nuclease were introduced into single-cell C57BL/6J zygotes and transferred to pseudopregnant females. Progeny were screened by DNA sequencing to identify correctly targeted pups, which were then bred to C57BL/6J mice for germline transmission. (J:304477)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tert (mouse) Summary of all recombinase alleles driven by Tert. Alliance mouse Tert gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tert Mutation: 55 strains or lines available

References

• Original: J:304477 Wei Y, et al., Liver homeostasis is maintained by midlobular zone 2 hepatocytes. Science. 2021 Feb 26;371(6532)
• All: 2 reference(s)