Tert^{tm4(cre/ERT2)Sear}
Targeted Allele Detail

Summary

• Symbol: Tert^{tm4(cre/ERT2)Sear}
• Name: telomerase reverse transcriptase; targeted mutation 4, Steven E Artandi
• MGI ID: MGI:6704920
• Synonyms: Tert^CreERT2
• Gene: Tert Location: Chr13:73775030-73797962 bp, + strand Genetic Position: Chr13, 40.12 cM
• Alliance: Tert^{tm4(cre/ERT2)Sear} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:261807
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tert^{tm4(cre/ERT2)Sear} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tert (MGI:1202709)

• Mutation details: The targeting vector was generated by inserting a codon-optimized intron-CreERT2-NeoR cassette into the endogenous translational start site of Tert in the BAC (RP24-342O18) via recombineering. The targeting vector was linearized and electroporated into JM8/F6 mouse ES cells. Correctly targeted ES clones were selected by Southern blotting and karyotypes, and then injected into ICR/CD-1 blastocysts to generate the knock-in line. (J:261807)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tert (mouse) Summary of all recombinase alleles driven by Tert. Alliance mouse Tert gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tert Mutation: 55 strains or lines available

References

• Original: J:261807 Lin S, et al., Distributed hepatocytes expressing telomerase repopulate the liver in homeostasis and injury. Nature. 2018 Apr;556(7700):244-248
• All: 2 reference(s)