Cacna1i^em1Jqp
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cacna1i^em1Jqp
• Name: calcium channel, voltage-dependent, alpha 1I subunit; endonuclease-mediated mutation 1, Jen Q Pan
• MGI ID: MGI:6704921
• Synonyms: Cacna1i^-
• Gene: Cacna1i Location: Chr15:80171439-80282480 bp, + strand Genetic Position: Chr15, 37.85 cM, cytoband E2
• Alliance: Cacna1i^em1Jqp page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology using sgRNA 5'-TCAGCCTCCGCACGGACAC-3' generated a 10 bp deletion (TCCCTGACAG) beginning at the codon corresponding to amino acid V756 resulting in a premature stop codon. Western blot analysis confirmed the absence of protein in homozygous brain. (J:302192)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cacna1i Mutation: 76 strains or lines available

References

• Original: J:302192 Ghoshal A, et al., Effects of a patient-derived de novo coding alteration of CACNA1I in mice connect a schizophrenia risk gene with sleep spindle deficits. Transl Psychiatry. 2020 Jan 23;10(1):29
• All: 2 reference(s)