Impact^tm1.1Casb
Targeted Allele Detail

Summary

• Symbol: Impact^tm1.1Casb
• Name: impact, RWD domain protein; targeted mutation 1.1, Beatriz A Castilho
• MGI ID: MGI:6710957
• Gene: Impact Location: Chr18:13088909-13126007 bp, + strand Genetic Position: Chr18, 6.87 cM, cytoband A2-B2
• Alliance: Impact^tm1.1Casb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:279071
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 3 and a loxP site and pgk-neo cassette were inserted downstream of exon 4 via homologous recombination. Recombinase mediated recombination removed exons 3 and 4. Western blot analysis confirmed the absence of protein expression in the heart, liver, and brain of homozygous mice. (J:279071)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Impact Mutation: 23 strains or lines available

References

• Original: J:279071 Pereira CM, et al., The GCN2 inhibitor IMPACT contributes to diet-induced obesity and body temperature control. PLoS One. 2019;14(6):e0217287
• All: 1 reference(s)