St8sia3^em2Yich
Endonuclease-mediated Allele Detail

Summary

• Symbol: St8sia3^em2Yich
• Name: ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3; endonuclease-mediated mutation 2, Yijuang Chern
• MGI ID: MGI:6711694
• Synonyms: St8sia3-^-, St8sia3-KO, St8sia3^tm2
• Gene: St8sia3 Location: Chr18:64387430-64409217 bp, + strand Genetic Position: Chr18, 37.25 cM
• Alliance: St8sia3^em2Yich page

Mutation origin

• Strain of Origin: C57BL/6JNarl

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 3 and exon/intron 4 were targeted with two sgRNAs using CRISPR/Cas9 technology, resulting in a 1966 bp deletion, encompassing most of exon 3 (which contains the ATG start site) and the entirety of intron 3 and exon 4, and 4 bp insertion. Immunoblots confirmed the absence of peptide expression from this allele in the brain. (J:285485)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any St8sia3 Mutation: 20 strains or lines available

References

• Original: J:285485 Lin CY, et al., Functional roles of ST8SIA3-mediated sialylation of striatal dopamine D2 and adenosine A2A receptors. Transl Psychiatry. 2019 Aug 27;9(1):209
• All: 1 reference(s)