Gt(ROSA)26Sor^{tm1(BEST1-cre/ERT2)Vold}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(BEST1-cre/ERT2)Vold}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Douglas Vollrath
• MGI ID: MGI:6711969
• Synonyms: Rosa26^{BEST1-CreERT2}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(BEST1-cre/ERT2)Vold} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:305495
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(BEST1-cre/ERT2)Vold} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  human

  BEST1 (7439)

  Best1

  (MGI:1346332)

• Mutation details: The allele was created to have the human BEST1 promoter/enhancer regions directing the expression of a CreER(T2) fusion gene. The targeting vector contained, from 5' to 3', the human BEST1 promoter (nucleotides -585 to +38), followed by a CreER(T2) fusion gene, and an SV40 a polyA cassette. The plasmid was pronuclear injected into C57BL/6J(attPx3) zygotes along with phiC31 integrase mRNA. The resulting founder mice were bred with C57BL/6J for germline transmission. (J:305495)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	BEST1 (human) Summary of all recombinase alleles driven by BEST1. Comparative matrix view of recombinase activities Alliance human BEST1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:305495 Chen M, et al., An efficient inducible RPE-Selective cre transgenic mouse line. Exp Eye Res. 2021 Jan;202:108370
• All: 1 reference(s)