Riok2^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Riok2^{tm1c(KOMP)Wtsi}
• Name: RIO kinase 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6712634
• Synonyms: Riok2^f
• Gene: Riok2 Location: Chr17:17594594-17615161 bp, + strand Genetic Position: Chr17, 9.77 cM, cytoband A3.1
• Alliance: Riok2^{tm1c(KOMP)Wtsi} page
• IMPC: Riok2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:305794
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 17603091 of Chromosome 17 upstream of the critical exons 5-6 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 5-6 at position 17605529. The critical exons 5 and 6 are thus flanked by loxP sites. Flp-mediated recombination removed the FRT-flanked cassette resulting in a "conditional read" allele in which exons 5-6 are floxed. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:305794)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Riok2 Mutation: 33 strains or lines available

References

• Original: J:305794 Raundhal M, et al., Blockade of IL-22 signaling reverses erythroid dysfunction in stress-induced anemias. Nat Immunol. 2021 Apr;22(4):520-529
• All: 3 reference(s)