Zfp639^tm1.2Jaty
Targeted Allele Detail

Summary

• Symbol: Zfp639^tm1.2Jaty
• Name: zinc finger protein 639; targeted mutation 1.2, John AT Young
• MGI ID: MGI:6713584
• Synonyms: ZASC^-
• Gene: Zfp639 Location: Chr3:32564408-32574982 bp, + strand Genetic Position: Chr3, 15.75 cM, cytoband B
• Alliance: Zfp639^tm1.2Jaty page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:305874
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An FRT site flanked puromycin resistance gene cassette and a loxP site were inserted into intron 3 and a second loxP site, an F3 site flanked neomycin resistance gene cassette and an EGFP reporter gene cassette were inserted downstream of exon 7. The puro and neo cassettes were removed through subsequent flp-mediated recombination. Floxed exons 4-7 were deleted through subsequent cre-mediated recombination, leaving a reporter null-allele. RT-PCR experiments confirmed the absence of transcription from this allele. (J:305874)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zfp639 Mutation: 19 strains or lines available

References

• Original: J:305874 Seidel S, et al., ZASC1 knockout mice exhibit an early bone marrow-specific defect in murine leukemia virus replication. Virol J. 2013 Apr 24;10:130
• All: 1 reference(s)