Setd4^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Setd4^{tm1b(KOMP)Wtsi}
• Name: SET domain containing 4; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:6715241
• Gene: Setd4 Location: Chr16:93380345-93400951 bp, - strand Genetic Position: Chr16, 54.52 cM
• Alliance: Setd4^{tm1b(KOMP)Wtsi} page
• IMPC: Setd4 gene page

Mutation origin

• Mutant Cell Line: EPD0225_5_E04
• Germline Transmission: Earliest citation of germline transmission: J:304151
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 93389900 of Chromosome 16 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 93390710. The critical exon(s) is/are thus flanked by loxP sites. Cre mediated recombination without prior flp expression created a reporter knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Setd4 Mutation: 30 strains or lines available

References

• Original: J:304151 Zhong Y, et al., The novel methyltransferase SETD4 regulates TLR agonist-induced expression of cytokines through methylation of lysine 4 at histone 3 in macrophages. Mol Immunol. 2019 Oct;114:179-188
• All: 3 reference(s)