Clu^{em1.1(cre/ERT2)Algr}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Clu^{em1.1(cre/ERT2)Algr}
• Name: clusterin; endonuclease-mediated mutation 1, Alex Gregorieff
• MGI ID: MGI:6716263
• Synonyms: Clu^cre-ERT2
• Gene: Clu Location: Chr14:66206093-66218992 bp, + strand Genetic Position: Chr14, 34.36 cM
• Alliance: Clu^{em1.1(cre/ERT2)Algr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:283362
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Clu^{em1.1(cre/ERT2)Algr} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Clu (MGI:88423)

• Mutation details: The allele was created by inserting the cre/ERT2 cassette at the translation start site of the mouse Clu locus in R1 ES cells using CRISPRCas9-mediated homologous recombination. The neomycin cassette was removed by crossing Clucre-ERT2 mice with Flp recombinase-expressing mice. (J:283362)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Clu (mouse) Summary of all recombinase alleles driven by Clu. Comparative matrix view of recombinase activities Alliance mouse Clu gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Clu Mutation: 32 strains or lines available

References

• Original: J:283362 Ayyaz A, et al., Single-cell transcriptomes of the regenerating intestine reveal a revival stem cell. Nature. 2019 May;569(7754):121-125
• All: 4 reference(s)