Foxp3^{tm4(EGFP/icre)Tch}
Targeted Allele Detail

Summary

• Symbol: Foxp3^{tm4(EGFP/icre)Tch}
• Name: forkhead box P3; targeted mutation 4, Talal A Chatila
• MGI ID: MGI:6717215
• Synonyms: Foxp3^{deltaEGFPiCre}
• Gene: Foxp3 Location: ChrX:7445915-7461482 bp, + strand Genetic Position: ChrX, 3.41 cM
• Alliance: Foxp3^{tm4(EGFP/icre)Tch} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:305614
• Parent Cell Line: SCC10 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Recombinase, Reporter)
• Mutation: Insertion
• Foxp3^{tm4(EGFP/icre)Tch} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Foxp3 (MGI:1891436)

• Mutation details: The targeting construct consisting of the IRES-EGFP-iCre sequence inserted immediately downstream of the Foxp3 translational stop codon and upstream of the endogenous polyadenylation signal and additional FRT flanked PGK-neo cassette inserted between exons 9-10 was used for homologous recombination in the ES cells. The PGK-neo cassette was removed by mating founder males with Flp-deleter female mice. Inserted Frt element inserted between exons 9 and 10 that created an aberrant 3 splice junction site, leading to a premature stop codon in the Foxp3 transcript. (J:305614)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Foxp3 (mouse) Summary of all recombinase alleles driven by Foxp3. Comparative matrix view of recombinase activities Alliance mouse Foxp3 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Foxp3 Mutation: 58 strains or lines available

References

• Original: J:305614 Charbonnier LM, et al., Functional reprogramming of regulatory T cells in the absence of Foxp3. Nat Immunol. 2019 Sep;20(9):1208-1219
• All: 6 reference(s)