Cd19^{em1.1(icre)Yoba}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cd19^{em1.1(icre)Yoba}
• Name: CD19 antigen; endonuclease-mediated mutation 1.1, Yoshihiro Baba
• MGI ID: MGI:6717340
• Synonyms: CD19-iCre
• Gene: Cd19 Location: Chr7:126007622-126014061 bp, - strand Genetic Position: Chr7, 69.01 cM, cytoband F3-F4
• Alliance: Cd19^{em1.1(icre)Yoba} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:304919
• Parent Cell Line: EGR-101 (ES Cell)
• Strain of Origin: C57BL/6NSlc

Mutation description

• Allele Type: Endonuclease-mediated (Recombinase)
• Mutation: Insertion
• Cd19^{em1.1(icre)Yoba} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cd19 (MGI:88319)

• Mutation details: The T2A-iCre sequence and neo selection cassette were inserted in-frame to the 3-end of the Cd19 coding sequence immediately upstream of the stop codon using CRISPR/Cas9-mediated homologous recombination technology. Flp-mediated recombination removed the selection cassette. (J:304919)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cd19 (mouse) Summary of all recombinase alleles driven by Cd19. Comparative matrix view of recombinase activities Alliance mouse Cd19 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd19 Mutation: 60 strains or lines available

References

• Original: J:304919 Yasuda T, et al., Generation and characterization of CD19-iCre mice as a tool for efficient and specific conditional gene targeting in B cells. Sci Rep. 2021 Mar 9;11(1):5524
• All: 1 reference(s)