Pfn4^em1Hsc
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pfn4^em1Hsc
• Name: profilin family, member 4; endonuclease-mediated mutation 1, Hubert Schorle
• MGI ID: MGI:6718278
• Synonyms: Pfn4deltaa
• Gene: Pfn4 Location: Chr12:4819022-4828813 bp, + strand Genetic Position: Chr12, 2.09 cM, cytoband A1.1
• Alliance: Pfn4^em1Hsc page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR-targeting of exons 2 through 4 deleted of 5417 bp leading to generation of a 5' truncated nonsense protein. qRT-PCR analysis confirmed the absence of mRNA expression in the testes of homozygous mutant males. (J:328252)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pfn4 Mutation: 18 strains or lines available

References

• Original: J:328252 Umer N, et al., PFN4 is required for manchette development and acrosome biogenesis during mouse spermiogenesis. Development. 2022 Aug 15;149(16):dev200499
• All: 1 reference(s)