Hbs1l^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Hbs1l^{tm1c(KOMP)Wtsi}
• Name: Hbs1-like (S. cerevisiae); targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6718578
• Synonyms: Hbs1l^fl
• Gene: Hbs1l Location: Chr10:21171876-21244788 bp, + strand Genetic Position: Chr10, 9.75 cM
• Alliance: Hbs1l^{tm1c(KOMP)Wtsi} page
• IMPC: Hbs1l gene page

Mutation origin

• Mutant Cell Line: EPD0693_2_A02
• Germline Transmission: Earliest citation of germline transmission: J:306384
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 21212311 of Chromosome 10 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 21213197. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:306384)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hbs1l Mutation: 51 strains or lines available

References

• Original: J:306384 Terrey M, et al., Defects in translation-dependent quality control pathways lead to convergent molecular and neurodevelopmental pathology. Elife. 2021 Apr 26;10:e66904
• All: 1 reference(s)