Pomc^{tm1.1(cre)Lowl}
Targeted Allele Detail

Summary

• Symbol: Pomc^{tm1.1(cre)Lowl}
• Name: pro-opiomelanocortin-alpha; targeted mutation 1.1, Bradford B Lowell
• MGI ID: MGI:6718623
• Synonyms: Pomc-IRES-Cre
• Gene: Pomc Location: Chr12:4004951-4010642 bp, + strand Genetic Position: Chr12, 1.99 cM
• Alliance: Pomc^{tm1.1(cre)Lowl} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:303221
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (No functional change, Recombinase)
• Mutation: Insertion
• Pomc^{tm1.1(cre)Lowl} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Pomc (MGI:97742)

• Mutation details: A selection cassette containing an internal ribosomal entry sequence (IRES) linked to Cre-recombinase and an Frt-flanked kanamycin resistance gene was targeted just downstream of the stop codon of the Pomc gene, so that Cre-recombinase expression was driven by the endogenous gene. Flpe recombinase transgene mice were then used to remove the kanamycin selection cassette. (J:303221)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Pomc (mouse) Summary of all recombinase alleles driven by Pomc. Comparative matrix view of recombinase activities Alliance mouse Pomc gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pomc Mutation: 21 strains or lines available

References

• Original: J:303221 Fenselau H, et al., A rapidly acting glutamatergic ARC-->PVH satiety circuit postsynaptically regulated by alpha-MSH. Nat Neurosci. 2017 Jan;20(1):42-51
• All: 4 reference(s)