Oprm1^{tm1.1(cre/ERT2)Dwata}
Targeted Allele Detail

Summary

• Symbol: Oprm1^{tm1.1(cre/ERT2)Dwata}
• Name: opioid receptor, mu 1; targeted mutation 1.1, Dai Watanabe
• MGI ID: MGI:6718628
• Synonyms: MOR-CreER
• Gene: Oprm1 Location: Chr10:6708593-6988209 bp, + strand Genetic Position: Chr10, 1.85 cM, cytoband A2
• Alliance: Oprm1^{tm1.1(cre/ERT2)Dwata} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:302775
• Parent Cell Line: EGR-101 (ES Cell)
• Strain of Origin: C57BL/6NSlc

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Oprm1^{tm1.1(cre/ERT2)Dwata} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Oprm1 (MGI:97441)

• Mutation details: A bacterial artificial chromosome (BAC) containing the mouse Oprm1 gene (RP24-112H16) was modified so that the translational stop codon in exon 4 of the Oprm1 gene was replaced by a cassette comprising the T2A sequence, icre/ERT2 gene with stop codon and FRT-flanked neomycin-resistant gene (T2A-iCreERT2-FRT-Neo-FRT). The homologous recombination in the mouse ES cells generated a chimeric mouse which was crossed with CAG-FLPe mice to remove the FRT-flanked neomycin resistance cassette in the offspring. (J:302775)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Oprm1 (mouse) Summary of all recombinase alleles driven by Oprm1. Comparative matrix view of recombinase activities Alliance mouse Oprm1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Oprm1 Mutation: 54 strains or lines available

References

• Original: J:302775 Okunomiya T, et al., Generation of a MOR-CreER knock-in mouse line to study cells and neural circuits involved in mu opioid receptor signaling. Genesis. 2020 Jan;58(1):e23341
• All: 1 reference(s)