Aurka^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Aurka^{tm1c(EUCOMM)Hmgu}
• Name: aurora kinase A; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6718894
• Gene: Aurka Location: Chr2:172198110-172212455 bp, - strand Genetic Position: Chr2, 94.84 cM
• Alliance: Aurka^{tm1c(EUCOMM)Hmgu} page
• IMPC: Aurka gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 172208620 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 172209537. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Aurka Mutation: 40 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 5 reference(s)