Vip^{tm1.1(cre)Pful}
Targeted Allele Detail

Summary

• Symbol: Vip^{tm1.1(cre)Pful}
• Name: vasoactive intestinal polypeptide; targeted mutation 1.1, Patrick M Fuller
• MGI ID: MGI:6718935
• Synonyms: VIP-IRES-Cre
• Gene: Vip Location: Chr10:5589218-5597617 bp, + strand Genetic Position: Chr10, 1.97 cM, cytoband A1
• Alliance: Vip^{tm1.1(cre)Pful} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:302732
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Vip^{tm1.1(cre)Pful} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Vip (MGI:98933)

• Mutation details: A selection cassette containing an internal ribosomal entry sequence (IRES) linked to cre and an Frt-flanked kanamycin gene was inserted 3bp downstream of the stop codon of the Vip gene contained in a BAC (RP24-216N8) using recombineering methods. This construct was used to isolate a targeting plasmid with the cre-kanamycin cassette with upstream and downstream Vip homology arms. ES cells were targeted and used for blastocyst injection. Chimeras were bred for germline transmission. The selection marker was excised by breeding to Flp deleter animals. (J:302732)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Vip (mouse) Summary of all recombinase alleles driven by Vip. Comparative matrix view of recombinase activities Alliance mouse Vip gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Vip Mutation: 24 strains or lines available

References

• Original: J:302732 Todd WD, et al., Suprachiasmatic VIP neurons are required for normal circadian rhythmicity and comprised of molecularly distinct subpopulations. Nat Commun. 2020 Sep 2;11(1):4410
• All: 1 reference(s)