Prdx5^{tm1.1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Prdx5^{tm1.1(KOMP)Vlcg}
• Name: peroxiredoxin 5; targeted mutation 1.1, Velocigene
• MGI ID: MGI:6719042
• Synonyms: Prdx5^-
• Gene: Prdx5 Location: Chr19:6884065-6887474 bp, - strand Genetic Position: Chr19, 5.08 cM
• Alliance: Prdx5^{tm1.1(KOMP)Vlcg} page
• IMPC: Prdx5 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:271141
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 2850bp between positions 6884133-6886982 of Chromosome 19 (Build GRCm39). The entire coding region of Prdx5 was replaced with a LacZ reporter gene and floxed neomycin resistance cassette. Subsequent cre expression excised the neomycin selection cassette. Western blot analysis confirmed the absence of protein expression in splenocytes from homozygous mutant mice. (J:136110, J:271141)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prdx5 Mutation: 19 strains or lines available

References

• Original: J:271141 Graham DB, et al., Nitric Oxide Engages an Anti-inflammatory Feedback Loop Mediated by Peroxiredoxin 5 in Phagocytes. Cell Rep. 2018 Jul 24;24(4):838-850
• All: 2 reference(s)