Gucy1b2^{tm4.1(cre)Mom}
Targeted Allele Detail

Summary

• Symbol: Gucy1b2^{tm4.1(cre)Mom}
• Name: guanylate cyclase 1, soluble, beta 2; targeted mutation 4.1, Peter Mombaerts
• MGI ID: MGI:6719255
• Synonyms: Gucy1b2-IRES-nlsCre-IRES-taulacZ
• Gene: Gucy1b2 Location: Chr14:62630125-62693738 bp, - strand Genetic Position: Chr14, 33.21 cM
• Alliance: Gucy1b2^{tm4.1(cre)Mom} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:307157
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Recombinase, Reporter)
• Mutation: Insertion
• Gucy1b2^{tm4.1(cre)Mom} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gucy1b2 (MGI:2660873)

• Mutation details: Sequence encoding an IRES followed by Cre recombinase with a nuclear localization signal followed by a second IRES and tau-lacZ followed by an FRT-flanked neomycin selection cassette was inserted immediately downstream of the stop codon, leaving the coding sequence intact with tri-cistronic expression resulting in the co-expression of nuclear-localized cre and the tau-lacZ reporter. Flp-mediated excision removed the neomycin selection cassette. (J:307157)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gucy1b2 (mouse) Summary of all recombinase alleles driven by Gucy1b2. Alliance mouse Gucy1b2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gucy1b2 Mutation: 33 strains or lines available

References

• Original: J:307157 Koike K, et al., Danger perception and stress response through an olfactory sensor for the bacterial metabolite hydrogen sulfide. Neuron. 2021 Jun;109:1-16
• All: 1 reference(s)