Gm10822^{Tn(pb-CAG-tdTomato,-cre)Cot2Zhu}
Transposon induced Allele Detail

Summary

• Symbol: Gm10822^{Tn(pb-CAG-tdTomato,-cre)Cot2Zhu}
• Name: predicted gene 10822; transposon insertion Cot2, Yuan Zhuang
• MGI ID: MGI:6720234
• Synonyms: Cot2
• Gene: Gm10822 Location: Chr2:73722752-73729402 bp, + strand Genetic Position: Chr2, 43.89 cM
• Alliance: Gm10822^{Tn(pb-CAG-tdTomato,-cre)Cot2Zhu} page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Transposon induced (Conditional ready, No functional change, Recombinase, Reporter)
• Mutation: Transposon insertion
• Gm10822^{Tn(pb-CAG-tdTomato,-cre)Cot2Zhu} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details: The transposon containing a CAG (CMV enhancer chicken beta Actin promoter beta-Globin first intron) promoter, F3 (an FRT spacer mutant), tdTomato, F13 (another FRT spacer mutant), second F3, Cre-IRES-GFP, second F13 followed by polyadenylation signal flanked by piggyBac-specific left and right terminal sequences was co-injected with a piggyBac-containing transgene to mobilize the transposon. CAG promoter drives constitutive expression of either the tdTomato marker or the Cre recombinase upon Flp/FRT-mediated recombination. The Cre cDNA is tagged at its 3 end with iresGFP for lineage tracing of Cre expression. The Tomato and Cre coding sequences are flanked by two distinct pairs of FRT sites with the internal FRT sites hopping over each other. Flp-induced recombination between either of the FRT pairs results in deletion of either Tomato or the Cre-GFP cassette, resulting in mutually exclusive expression of either Cre-GFP or tdTomato, respectively. Lines with single-copy integration events outside any essential genes were screened by crossing the founder mouse to a piggyBac transposase expression strain and screened for new insertion events in the F2 generation. Three independent insertional events on separate chromosomes were identified and one Line named Cot2 inserted into the intron of the GM10822 locus (Ch2: 76405893 Ensemble version 99) in cis orientation was established for further analysis. (J:297537)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	CAG (chicken) Summary of all recombinase alleles driven by CAG.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gm10822 Mutation: 20 strains or lines available

References

• Original: J:297537 Wang Q, et al., A mosaic analysis system with Cre or Tomato expression in the mouse. Proc Natl Acad Sci U S A. 2020 Nov 10;117(45):28212-28220
• All: 1 reference(s)