Npvf^{tm1.1(icre)Gand}
Targeted Allele Detail

Summary

• Symbol: Npvf^{tm1.1(icre)Gand}
• Name: neuropeptide VF precursor; targeted mutation 1.1, Greg M Anderson
• MGI ID: MGI:6720253
• Synonyms: RFRP-Cre
• Gene: Npvf Location: Chr6:50627652-50631419 bp, - strand Genetic Position: Chr6, 24.34 cM, cytoband B3
• Alliance: Npvf^{tm1.1(icre)Gand} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:300737
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Npvf^{tm1.1(icre)Gand} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Npvf (MGI:1926488)

• Mutation details: Using homologous recombination in mouse embryonic stem cells, an internal ribosomal entry sequence (IRES) linked to an improved cre recombinase gene and an FRT-flanked neomycin resistance gene was targeted into the 3' untranslated region immediately downstream of the termination codon within the Npvf gene. FLPe-deleter mice were crossed to remove the FRT-flanked neomycin selection cassette. (J:300737)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Npvf (mouse) Summary of all recombinase alleles driven by Npvf. Comparative matrix view of recombinase activities Alliance mouse Npvf gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Npvf Mutation: 5 strains or lines available

References

• Original: J:300737 Mamgain A, et al., RFamide-Related Peptide Neurons Modulate Reproductive Function and Stress Responses. J Neurosci. 2021 Jan 20;41(3):474-488
• All: 1 reference(s)