Irs4^{tm1.1(cre)Dolsn}
Targeted Allele Detail

Summary

• Symbol: Irs4^{tm1.1(cre)Dolsn}
• Name: insulin receptor substrate 4; targeted mutation 1.1, David P Olson
• MGI ID: MGI:6720261
• Synonyms: IRS4-iCre
• Gene: Irs4 Location: ChrX:140493994-140508213 bp, - strand Genetic Position: ChrX, 62.43 cM
• Alliance: Irs4^{tm1.1(cre)Dolsn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:299851
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Irs4^{tm1.1(cre)Dolsn} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Irs4 (MGI:1338009)

• Mutation details: Using a BAC recombineering method, a targeting construct containing an insertion of an Frt-flanked neomycin selection cassette followed by an internal ribosomal entry (tires) sequence fused to a Cre recombinase transgene (iCre) between the STOP codon and the polyadenylation of the Irs4 gene. This construct was used to isolate a targeting plasmid with the neo-iCre cassette with upstream and downstream Crh homology arms. ES cells were targeted and used for blastocyst injection. Chimeras were bred for germline transmission. The selection marker was excised by breeding to Flp deleter animals. (J:299851)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Irs4 (mouse) Summary of all recombinase alleles driven by Irs4. Comparative matrix view of recombinase activities Alliance mouse Irs4 gene page

Expression

In Mice Carrying this Mutation:

2 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Irs4 Mutation: 5 strains or lines available

References

• Original: J:299851 Sutton AK, et al., Paraventricular, subparaventricular and periventricular hypothalamic IRS4-expressing neurons are required for normal energy balance. Sci Rep. 2020 Mar 26;10(1):5546
• All: 1 reference(s)