Tbr1^{tm1(cre/ERT2)Zjh}
Targeted Allele Detail

Summary

• Symbol: Tbr1^{tm1(cre/ERT2)Zjh}
• Name: T-box brain transcription factor 1; targeted mutation 1, Z Josh Huang
• MGI ID: MGI:6720839
• Synonyms: Tbr1-2A-CreER, Tbr1-CreER
• Gene: Tbr1 Location: Chr2:61633274-61644458 bp, + strand Genetic Position: Chr2, 35.56 cM
• Alliance: Tbr1^{tm1(cre/ERT2)Zjh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:323692
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tbr1^{tm1(cre/ERT2)Zjh} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tbr1 (MGI:107404)

• Mutation details: The allele was generated by inserting a 2A-CreER expression cassette in-frame before the STOP codon of the Tbr1 gene. The targeting vectors generated using BAC clones from the RPCI-23&24 library were electroporated into a 129SVj/B6 hybrid ES cell line (v6.5). The Resulting ES cell clones were used for tetraploid complementation to obtain male heterozygous mice. (J:323692)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tbr1 (mouse) Summary of all recombinase alleles driven by Tbr1. Alliance mouse Tbr1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tbr1 Mutation: 38 strains or lines available

References

• Original: J:323692 Matho KS, et al., Genetic dissection of the glutamatergic neuron system in cerebral cortex. Nature. 2021 Oct;598(7879):182-187
• All: 1 reference(s)