Btg2^{tm1.1(cre/ERT2)Zjh}
Targeted Allele Detail

Summary

• Symbol: Btg2^{tm1.1(cre/ERT2)Zjh}
• Name: BTG anti-proliferation factor 2; targeted mutation 1.1, Z Josh Huang
• MGI ID: MGI:6720842
• Synonyms: Tis21-2A-CreER, Tis21-CreER
• Gene: Btg2 Location: Chr1:134002908-134006858 bp, - strand Genetic Position: Chr1, 58.1 cM
• Alliance: Btg2^{tm1.1(cre/ERT2)Zjh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:323692
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Btg2^{tm1.1(cre/ERT2)Zjh} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Btg2 (MGI:108384)

• Mutation details: The allele was generated by inserting a 2A-CreER expression cassette in-frame before the STOP codon of the Btg2 gene. The targeting vectors generated using BAC clones from the RPCI-23&24 library were electroporated into a 129SVj/B6 hybrid ES cell line (v6.5). The Resulting ES cell clones were used for tetraploid complementation to obtain male heterozygous mice. The neo selection cassette was excised by crossing to FlpE strain mice. (J:323692)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Btg2 (mouse) Summary of all recombinase alleles driven by Btg2. Comparative matrix view of recombinase activities Alliance mouse Btg2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Btg2 Mutation: 16 strains or lines available

References

• Original: J:323692 Matho KS, et al., Genetic dissection of the glutamatergic neuron system in cerebral cortex. Nature. 2021 Oct;598(7879):182-187
• All: 2 reference(s)