Eomes^{tm2.1(flpo/ERT2)Zjh}
Targeted Allele Detail

Summary

• Symbol: Eomes^{tm2.1(flpo/ERT2)Zjh}
• Name: eomesodermin; targeted mutation 2.1, Z Josh Huang
• MGI ID: MGI:6720888
• Synonyms: Tbr2-FlpER
• Gene: Eomes Location: Chr9:118307259-118315176 bp, + strand Genetic Position: Chr9, 70.21 cM, cytoband F3
• Alliance: Eomes^{tm2.1(flpo/ERT2)Zjh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:323692
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Eomes^{tm2.1(flpo/ERT2)Zjh} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Eomes (MGI:1201683)

• Mutation details: The allele was generated by inserting a 2A-flpoER expression cassette in-frame before the STOP codon of the Eomes gene. The targeting vectors generated using BAC clones from the RPCI-23&24 library were electroporated into a 129SVj/B6 hybrid ES cell line (v6.5). The Resulting ES cell clones were used for tetraploid complementation to obtain male heterozygous mice. The neo selection cassette was excised by crossing to cre expressing mice. (J:323692)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Eomes (mouse) Summary of all recombinase alleles driven by Eomes. Alliance mouse Eomes gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Eomes Mutation: 44 strains or lines available

References

• Original: J:323692 Matho KS, et al., Genetic dissection of the glutamatergic neuron system in cerebral cortex. Nature. 2021 Oct;598(7879):182-187
• All: 1 reference(s)